Lysozyme Diffuse Data Processing

Testing page for Experiment Registration

Overview

Processed diffuse scattering data including 3D diffuse maps and integrated Bragg intensities.

Input Data

• Lysozyme Diffuse Scattering Dataset (raw CBF files)

Processing Steps

1. Image processing and background subtraction
2. Bragg peak integration using XDS
3. Diffuse scattering extraction
4. 3D diffuse map construction
5. Symmetry averaging

Results

• Structure factors: lysozyme_merged.mtz (99.8% complete to 1.5 Å)
• Diffuse maps: lysozyme_diffuse_3d.h5 (HDF5 format)
• Statistics: Rmerge = 6.2%, CC1/2 = 0.998
• Quality: Excellent agreement between symmetry-related observations

Analysis

Strong diffuse scattering patterns observed along specific crystallographic directions, indicating correlated molecular motions. Patterns consistent with liquid-like motions previously reported for lysozyme.

Next Steps

• Molecular dynamics simulation comparison
• Anisotropic displacement parameter refinement
• Publication of results